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In our studies two primer pairs within the VP2 protein gene of canine parvovirus were chosen on the base of the sequence of Polish isolates. The sensitivity of detection of canine parvovirus in stool samples by Nested PCR was increased 60% in comparison with the standard PCR method and it was 30% higher than the virus isolation in tissue culture. A simple procedure of sample preparation with the use...
One hundred and seventy-six samples collected from internal organs of sick birds and 40 samples taken from healthy birds were used for virus isolation. The isolation was carried out on SPF chicken embryos by inoculating them into the yolk sac with the material prepared by generally accepted methods. Three passages were performed. The presence of viral antigen was detected by the agar gel precipitation...
Several components of sheep blood extensively catabolize nucleosides, including thymidine. Part of the radioactivity from tritiated thymidine, in whole blood cultures, is quickly rendered unincorporable as the compound is metabolized to thymine and further breakdown products. Thus, cells continue to proliferate without incorporating radioactivity from the medium. Furthemore, variability in the degree...
The investigations were carried out on 75 infertile cows with cystic ovarian disease, and 25 control cows with corpus luteum. The blood concentrations of ketone bodies, free fatty acids, glucose, total cholesterol and progesterone were determined before cyst treatment with GnRH. Levels of ketone bodies and free fatty acids were higher and progesterone level was lower in cystic cows than in control...
A total of 197 samples of MDPM (158 chicken, 31 turkey and 8 goose samples) was measured. Mean total phosphorus contents (X ± SD) in MDPM from chickens, turkeys and geese were 0.163%, 0.187%, and 0.080%, respectively. Mean phosphorus concentrations in poultry sausages, poultry frankfurters and pies and roasts were 0.168%, 0.151%, and 0.136%, and the contents of phosphorus calculated as added were...
Immunogenic effects of the oral inactivated E.coli vaccine given to 3-4-week-old piglets were studied. The vaccine consisting of E.coli strain 0149:K91, expressing the fimbrial colonization antigen F4ac supplemented with a toxoid (CTB) and CT was administered in two or three doses, respectively. The local gut antibody responses in fecal samples and in the intestinal mucosa were tested by ELISA. It...
The aim of the studies was to determine the influence of sex on the level of selected blood haematological and biochemical indices in 5-month-old Polish Longwool lambs. The studies pointed to higher levels of the Ht indices and Hb content (P≤0.01 ), activity of ALT and AST transaminase, Mg, Fe, total iron binding capability and total protein (P≤0.01), and a lower level of Cu (P≤0.01) in the blood...
Protection of weaned piglets against E.coli diarrhea depends on specific antibacterial and/or antitoxic antibodies induced by oral immunization. A formalin-inactivated vaccine, consisting of E.coli strain O149:K91 expressing the F4ac fimbrial antigen and B subunit of CT was orally administered to 3 - 4-week-old pigs. The serum anti-LPS, anti-F4ac, and anti-CTB antibodies were tested by ELISA. It was...
A long-term nitrate load was given to calves by oral administration of aqueous solution of KNO₃ to 6 five-week-old experimental calves. The calves were divided into two groups, three animals in each. During a period of six weeks the calves in the experimental group 1, received 2 g of KNO₃ every day, while those of the 2nd group were administered 5 g of KNO₃ per day. After 6 week application, when...
The studies were carried out on carps harvested from 10 various ponds in various seasons or the year. Several fish from each pond were infected with the pathogenic strain Aeromonas hydrophila and the number of diseased and dead fish and the signs or disease intensification in fish from each pond were evaluated. Other fish collected from the same ponds were subjected to number or thrombocytes, the...
The period of swine vesicular disease (SVD) virus persistence in clinical and tissue samples from experimentally infected pigs was investigated. A range of samples including epithelial tissue from vesicles, nasal swabs, blood, faeces and organs were collected to examine for the presence of infectious SVD particles, genomes and antigen. For this purpose, the conventional and PCR techniques were applied...
The aim of our study was to observe the contents of nitrates and nitrites during the six stages of manufacture of Edam cheese. Samples were taken of raw milk, of pasteurized milk, of milk with nitrates added, of pressed cheese curd, of whey, of ripening cheese and the final product. The samples were drawn from a commercial operation in cheese factory in the eastern part of Slovakia. The mean natura...
Three groups of carps were kept for 6 to 12 weeks at 11°C, 16°C and 23°C. A portion of the fish from each group was infected with Aeromonas hydrophila. Experimental fish were subjected to evaluation of number of thrombocytes, the index of bacteria-thrombocyte adherence (IB-TA), the percentage of active thrombocytes (%AT) and the index of thrombocyte aggregation (ITA). Carps kept at 11℃ revealed the...
Bacteriostatic activity against Staphylococcus aureus of eleven single flavonoids, pinostrobin chalcone, cinnamic acid and four of its derivatives, hydrocinnamic acid, benzoic acid and six of its derivatives and vanillin occurring in propolis has been determined quantitatively by the method of serial dilutions. Moreover, the determination of bacteriostatic activity has been performed using ethanolic...
The aim of the studies was to compare the Ht values and the content of Hb in blood, total protein. Na, K, Ca, inorganic P and Mg in blood serum and bone tissue in lambs born in different seasons. A higher (P≤0.05) Ht value, K and Ca content, and a lower total protein, Na, inorganic P and Mg content were found in the blood of lambs born in autumn. A higher (P≤0.05) content of Ca and Mg, but a lower...
The aim of these studies was to determine the performances of five commercial ELISA kits in the detection of BLV antibodies in serum samples. Different variants of ELI SA methods (indirect, blocking and double-well) were employed for these kits. Comparative investigations using 428 serum samples showed 418 consistent results (97.7%). Ten results (2.3%) proved to be inconsistent including 7 (1.6%)...
The applicability of the polymerase chain reaction (PCR) for the detection of proviral DNA of BLV in newborn calves was tested. Out of 22 calves from BLV infected mothers, studied during first 3 weeks after birth, 13 were serologically positive by ELISA. However, only 3 calves were positive for BL V provirus in their blood leukocytes’ DNA. Since the serological test can not discriminate active infection...
The results of PCR were compared with the results of virus isolation test. 44 bovine semen samples were tested. The presence of BHV 1 was demonstrated with the virus isolation test in 6 samples of semen. PCR with external primers detected BHV 1 in 8 samples, while in nested PCR positive amplification was observed in 21 samples. The sensitivity of PCR with external primers and nested PCR were 0.6 and...
The effects of iv infusion of 7.2% NaCl (HSS, 4 ml/kg; n=6) or 7.2% NaCl in 6% dextran 70 (HSD, 4 ml/kg; n=6) followed by administration of 0.9% NaCl (ISS, 32 ml/kg) on sodium (Na), chloride (Cl), potassium (K) serum concentrations, osmolality (Osm), total protein content (TP), packed cell volume (PCV), haemoglobin concentration (Hb) and red blood cell count (RBC) were evaluated in twelve healthy...
Evaluation of the immunogenicity of the vaccine against respiratory tract infections in calves caused by Pasteurella multocida, and prepared from antigen of a local strain of serotype 3 with oil adjuvant addition, was the object of the prescnt studies. Two experiments were carried out using calves. Vaccine was given them at a dose of 2 ml i.m., twice every 14 days. In experiment 1, sera were obtained...
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